Elevate your diagnostic capabilities with the Bio-Rad QX200 Droplet Digital PCR (ddPCR) System. This CE-IVD marked system offers highly accurate detection and quantification of nucleic acids, aiding medical practitioners in clinical decision-making for diseases ranging from cancer to transplant rejection and viral infection. The system includes a QX200 Droplet Generator, QX200 Droplet Reader, and QuantaSoft software, all CE-IVD marked for in vitro diagnostics in the European Union. 

These instruments are the only digital PCR systems available for clinical laboratory use. The QX200 Droplet Generator partitions ddPCR reaction mixtures into thousands of nanoliter-sized droplets, which are individually analyzed by the QX200 Droplet Reader after endpoint PCR. By counting PCR-positive and PCR-negative droplets, the system provides absolute quantification of target DNA, ensuring precise and reliable diagnostic results. Trust Bio-Rad QX200 for advanced molecular diagnostics in clinical settings.

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Question & Answers For:

Bio-Rad QX200

Past Questions, Helpful Answers.

q.

What is the primary purpose of the Bio-Rad QX200 Droplet Digital PCR System?

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The Bio-Rad QX200 system is designed for highly accurate detection and quantification of nucleic acids. It is mainly used in clinical diagnostics to aid in detecting and quantifying DNA and RNA, supporting medical decisions for conditions like cancer, viral infections, and transplant rejection.

 

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How does the Bio-Rad QX200 Droplet Digital PCR System ensure accuracy in its results?

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The QX200 system partitions samples into thousands of nanoliter-sized droplets, each undergoing PCR individually. By analyzing each droplet for PCR-positive or PCR-negative status, the system provides absolute quantification of target DNA, minimizing variability and ensuring precise results.

 

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Is the Bio-Rad QX200 system suitable for clinical laboratory use?

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Yes, the Bio-Rad QX200 is CE-IVD marked, indicating it is approved for in vitro diagnostics in the European Union, making it suitable and validated for clinical laboratory use.

 

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What are the main components of the Bio-Rad QX200 system?

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The Bio-Rad QX200 system includes three key components: the QX200 Droplet Generator, the QX200 Droplet Reader, and the QuantaSoft software. Each of these components is CE-IVD marked for clinical applications.

 

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Can the Bio-Rad QX200 system be used for both DNA and RNA quantification?

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Yes, the Bio-Rad QX200 system is capable of quantifying both DNA and RNA, making it versatile for a range of diagnostic applications in clinical settings.

 

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How does the QX200 Droplet Generator work in the system?

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The QX200 Droplet Generator takes the PCR reaction mixture and partitions it into thousands of tiny droplets, each acting as an individual reaction vessel. This partitioning is essential for achieving the high sensitivity and precision of the digital PCR process.

 

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What software is included with the Bio-Rad QX200 system, and what is its purpose?

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The system includes QuantaSoft software, which is used to analyze the data from each droplet. It provides the absolute quantification data required for accurate interpretation of the PCR results in clinical diagnostics.

 

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Why is digital PCR technology beneficial in clinical diagnostics?

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Digital PCR technology provides absolute quantification, meaning it directly counts target molecules rather than estimating them, which improves accuracy and sensitivity. This high precision is particularly beneficial in detecting low-level nucleic acids in challenging clinical samples.

 

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Does the Bio-Rad QX200 require any special training to operate?

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While the system is designed for clinical use, users may benefit from training to fully understand the ddPCR process and software analysis. Bio-Rad typically offers resources or training to help laboratory staff get the most out of the system.

 

q.

Is the Bio-Rad QX200 compatible with other laboratory instruments?

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The Bio-Rad QX200 system is intended to function as a standalone digital PCR solution for precise nucleic acid quantification. However, data output can often be integrated into laboratory information management systems (LIMS) depending on lab setup.