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Waters HPLC Pump
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Question & Answers For:
Waters HPLC Pump
Past Questions, Helpful Answers.
q.
What is an HPLC pump used for?
a.
An HPLC pump is used in High-Performance Liquid Chromatography (HPLC) systems to deliver a precise and consistent flow of liquid mobile phase through the chromatography column, enabling accurate separation of chemical compounds.
q.
What types of HPLC pumps are available?
a.
HPLC pumps generally fall into three categories: isocratic pumps (single solvent at a constant flow rate), gradient pumps (mix multiple solvents in varying proportions), and quaternary pumps (allow for more complex solvent mixing).
q.
What flow rate range does a Waters HPLC Pump support?
a.
The flow rate depends on the specific model, but Waters HPLC pumps typically support a range from microliters per minute to milliliters per minute, suitable for analytical and preparative chromatography applications.
q.
Is a Waters HPLC pump compatible with different solvents?
a.
Yes, Waters HPLC pumps are designed to handle a variety of solvents used in chromatography, but it is important to check the chemical compatibility of seals and tubing with the solvents being used.
q.
How do I maintain a Waters HPLC pump for optimal performance?
a.
Regular maintenance includes flushing the pump with appropriate solvents, replacing worn-out seals and check valves, and ensuring proper calibration to maintain consistent flow rates.
q.
Can I use a Waters HPLC pump for both analytical and preparative chromatography?
a.
Some Waters HPLC pumps are designed for analytical applications, while others can handle higher flow rates and pressures suitable for preparative chromatography. Check the specifications of the specific model to confirm suitability.
q.
What factors should I consider before selecting a Waters HPLC pump?
a.
Key factors include the type of chromatography application (analytical or preparative), required flow rate and pressure, solvent compatibility, and whether an isocratic or gradient system is needed.