Pharmacia FPLC System

The Pharmacia FPLC System is used for the automated separation and purification of proteins. It is ideal for semi-preparative amounts and ensures biological activity is preserved during purification by operating at low temperatures (4°C).

The system is designed to purify a wide variety of proteins using aqueous buffers. It is particularly useful for proteins requiring high specificity and gentle separation conditions.

The system supports five different chromatography columns: Superdex 75 and Superose 12 (gel filtration), Mono Q (anion exchange), Mono S (cation exchange), and phenyl sepharose (hydrophobic interaction).

The Pharmacia FPLC System features a "cold box" that maintains a constant temperature of 4°C during runs, which helps preserve the biological activity of proteins.

Yes, the Pharmacia FPLC System features two pumps for gradient elutions, providing precise control over the separation process.

Yes, the Pharmacia FPLC System includes an automated fraction collector, allowing for hands-free collection of protein fractions during the purification process.

The system is ideal for semi-preparative protein amounts, typically used for moderate-scale purification. It is optimized for precision, not for large-scale industrial use.

The Pharmacia FPLC System offers automated purification with precise control over temperature and gradients, making it highly efficient and suitable for maintaining protein activity during separation.

Yes, the system supports both anion exchange chromatography (using Mono Q) and cation exchange chromatography (using Mono S), making it versatile for different types of protein separations.

Regular maintenance involves cleaning the system after each use, ensuring proper calibration, and checking the pumps and fraction collector for functionality. Specific maintenance guidelines will be available in the user manual.