What is the main advantage of UHPLC over HPLC?

UHPLC systems operate at higher pressures, allowing for faster analysis, improved resolution, and greater sensitivity compared to traditional HPLC systems.

What types of samples can be analyzed using a UHPLC system?

UHPLC systems are ideal for analyzing small molecules, biomolecules, peptides, proteins, and pharmaceuticals, as well as food and environmental samples.

What factors should I consider when selecting a UHPLC system?

Key factors include pressure capacity, flow rate control, column compatibility, method development options, and integration with existing laboratory workflows.

Can I use my existing HPLC methods on a UHPLC system?

Many UHPLC systems are compatible with HPLC methods, but adjustments may be required due to differences in column particle size and system pressure.

What type of detectors can be used with UHPLC systems?

UHPLC systems support various detectors, including UV-Vis, fluorescence, mass spectrometry (MS), refractive index (RI), and electrochemical detectors.

Do UHPLC systems require special columns?

Yes, UHPLC systems use columns with smaller particle sizes (typically sub-2 µm) to achieve higher efficiency and resolution.

How does pressure capacity affect UHPLC performance?

Higher pressure capacity enables the use of smaller particle size columns, which improves separation efficiency and reduces analysis time.

Is a UHPLC system suitable for high-throughput analysis?

Yes, UHPLC systems are designed for high-speed analysis, making them well-suited for high-throughput laboratories.

UHPLC Systems Equipment, Parts and Supplies

Discover a variety of new, used, and refurbished Ultra-High Performance Liquid Chromatography (UHPLC) systems available for purchase online. UHPLC Systems offer enhanced resolution and performance through increased pressure capabilities, making them ideal for the separation and quantitation of small molecules, biomolecules, and peptides.

When selecting a UHPLC system, consider key metrics such as pressure capacity, flow rate control, method development options, and compatibility with existing methods. Explore trusted vendors to find high-quality UHPLC systems that meet your laboratory’s specific needs and budget.